Radiometric measurement of primary (PP) and secondary production (SP) in Rotmoosferner cryoconites sampled for metagenome sequencing on 14 September 2010 Arwyn Edwards Justin A Pachebat Martin Swain Matt Hegarty Andrew J Hodson Tristram D L Irvine-Fynn Sara M E Rassner Birgit Sattler 10.6084/m9.figshare.1011668.v1 https://iop.figshare.com/articles/dataset/___Radiometric_measurement_of_primary_PP_and_secondary_production_SP_in_Rotmoosferner_cryoconites_sa/1011668 <p><b>Table 1.</b>  Radiometric measurement of primary (PP) and secondary production (SP) in Rotmoosferner cryoconites sampled for metagenome sequencing on 14 September 2010. Values are the mean of triplicate incubations, and cryoconites with a positive ratio of SP:PP consistent with net heterotrophy are highlighted in bold. </p> <p><strong>Abstract</strong></p> <p>Cryoconite is a microbe–mineral aggregate which darkens the ice surface of glaciers. Microbial process and marker gene PCR-dependent measurements reveal active and diverse cryoconite microbial communities on polar glaciers. Here, we provide the first report of a cryoconite metagenome and culture-independent study of alpine cryoconite microbial diversity. We assembled 1.2 Gbp of metagenomic DNA sequenced using an Illumina HiScanSQ from cryoconite holes across the ablation zone of Rotmoosferner in the Austrian Alps. The metagenome revealed a bacterially-dominated community, with <em>Proteobacteria</em> (62% of bacterial-assigned contigs) and <em>Bacteroidetes</em> (14%) considerably more abundant than <em>Cyanobacteria</em> (2.5%). Streptophyte DNA dominated the eukaryotic metagenome. Functional genes linked to N, Fe, S and P cycling illustrated an acquisitive trend and a nitrogen cycle based upon efficient ammonia recycling. A comparison of 32 metagenome datasets revealed a similarity in functional profiles between the cryoconite and metagenomes characterized from other cold microbe–mineral aggregates. Overall, the metagenomic snapshot reveals the cryoconite ecosystem of this alpine glacier as dependent on scavenging carbon and nutrients from allochthonous sources, in particular mosses transported by wind from ice-marginal habitats, consistent with net heterotrophy indicated by productivity measurements. A transition from singular snapshots of cryoconite metagenomes to comparative analyses is advocated.</p> 2013-07-03 00:00:00 cryoconite ecosystem alpine glacier metagenome sequencing alpine cryoconite Abstract Cryoconite ice surface sp 14 September 2010. Values 32 metagenome datasets Functional genes nitrogen cycle Illumina HiScanSQ Microbial process ammonia recycling eukaryotic metagenome cryoconite holes ablation zone triplicate incubations cryoconite metagenome allochthonous sources productivity measurements Rotmoosferner cryoconites pp Austrian Alps metagenomic snapshot P cycling cryoconite metagenomes metagenomic DNA Environmental Science